Composite
Part:BBa_K660003
Designed by: Glasgow Uni 2011 Group: iGEM11_Glasgow (2011-09-16)
iLOV + RBS + TT
A composite part featuring the iLOV biobrick (https://parts.igem.org/Part:BBa_K660004) with an RBS (https://parts.igem.org/Part:BBa_B0030) and double terminator (https://parts.igem.org/Part:BBa_B1006).
iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness.
As a reporter, it is advantageous over GFP derived fluorescent proteins for a number of reasons. Firstly, it is small in size so can be used when tagging proteins. It also recovers quickly from photobleaching. Uniquely, it has the ability to function in anoxic conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |